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Image Search Results
Journal: Molecules
Article Title: Antileishmanial and Cytotoxic Compounds from Valeriana wallichii and Identification of a Novel Nepetolactone Derivative
doi: 10.3390/molecules20045740
Figure Lengend Snippet: Overview of the bioassay-guided fractionation of V. wallichii extract. Method A : preparative HPLC (250 mm × 10 mm, 5 µm, Nucleosil 100-5); H 2 O (a): MeOH (b), gradient: 70% b (0 min), 75% b (7 min), 100% b (25 min), 70% b (30 min); flow rate: 3.3 mL/min. Method B : preparative HPLC (125 mm × 10 mm, 5 µm, Nucleodur Sphinx RP); H 2 O (a): CH 3 CN (b), gradient: 10% b (0–2 min), 30% b (2–15 min), 65% b (15–22 min), 10% b (22–24 min); flow rate: 4.4 mL/min. Method C : flash chromatography (silica gel; MeOH/CHCl 3 , 4.8:0.2, v/v ). Method D : column chromatography (silica gel; MeOH/CHCl 3 , 4.8:02, v/v ).
Article Snippet: A portion of 600 mg of fraction F1 was further fractionated by
Techniques: Bioassay, Fractionation, Chromatography, Column Chromatography